We are ready to help you overcome the challenges of producing and developing innovative biotechnological products and advancing scientific research
Our company provides comprehensive recombinant protein design services focused on ensuring high-level expression, solubility, and functionality in bacterial and yeast systems. By integrating expertise in molecular biology, bioinformatics, and protein engineering, we deliver optimized constructs and expression strategies tailored to each client’s research or production goals
We specialize in custom vector construction and multi-gene cloning solutions to meet diverse expression requirements. Our team designs and optimizes constructs utilizing a broad portfolio of industry-standard bacterial and yeast expression vectors, including:
Bacterial Vectors: pET, pGEX, pCold, pBAD, pACYCDuet, pRSFDuet, pCDFDuet, and pMAL
Yeast Vectors: pPICZ, pGAPZ, pYES, and pESC
To streamline downstream purification and enhance protein solubility, we strategically incorporate a variety of fusion and affinity tags:
Affinity Tags: His₆, Strep-tag II, FLAG, HA, and c-Myc
Solubility Tags: GST, MBP, SUMO, NusA, and TrxA
Specific Protease Cleavage Sites: TEV, HRV 3C, or thrombin recognition sites for efficient tag removal.
We also design systems for co-expression, enabling simultaneous production of multiple subunits, enzyme cascades, or dedicated chaperone complexes. Our advanced cloning strategies—including Gibson Assembly, Golden Gate, and Ligation-Independent Cloning (LIC)—ensure the generation of accurate, efficient, and ready-to-express constructs. Every design undergoes rigorous in silico validation prior to gene synthesis.
We offer complete codon optimization services specifically for E. coli and yeast expression systems. Each target gene is systematically optimized based on host-specific codon usage, GC content, and predicted mRNA stability. We strategically remove cryptic restriction sites, spurious regulatory motifs, and detrimental secondary structures to maximize translational efficiency and ensure consistent, high-yield protein production.
We meticulously evaluate the characteristics of each target protein to determine the optimal expression host and strategy. This optimization includes fine-tuning the promoter and ribosomal binding site (RBS), selecting appropriate signal peptides for targeted periplasmic or extracellular secretion, and calibrating key culture and induction parameters. Our objective is clear: to maximize the yield of soluble and functionally active protein within the chosen bacterial or yeast platform
For highly challenging proteins, we offer custom molecular chaperone co-expression services. Utilizing advanced in silico analysis of folding pathways and aggregation hotspots, we identify or engineer highly effective chaperones, including canonical systems like GroEL/ES, DnaK/DnaJ, Trigger Factor, and ClpB. Furthermore, we can design novel fusion-based or synthetic chaperone systems precisely tailored to the client’s specific protein, robustly promoting correct folding and maximizing the recovery of active, soluble product
Our experts provide comprehensive technical support and consultation, guiding clients seamlessly from initial construct design through expression optimization and final purification. We are equipped to troubleshoot complex expression challenges and develop scalable, cost-effective systems essential for high-quality recombinant protein production
+98 912 836 0916
+98 930 144 1004
nimanezhad86@gmail.com
Custom-optimized rational enzyme engineering for your process requirements
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